John W. Newman

 

Post-Doctoral Researcher

Tel: (530) 752-0312

Fax: (530) 752-1537

email:

Education

PhD, Pharmacology and Toxicology, UC Davis, April 2002.
B.A., Biochemistry and Molecular Biology, UC Santa Cruz, June 1991.

Research

• Investigation of biochemical pathways associated with the oxidative transformation of arachadonic and linoleic acids by cytochrome P450 and soluble epoxide hydrolase (sEH).
• Development of novel analytical techniques to quantify epoxy and dihydroxy fatty acids derived from both linoleic and arachidonic acids using an array of liquid and gas chromatography/mass spectrometry methods.
• Quantitative analysis of epoxide and diol products as associated with the regulation of cardiovascular physiology and inflammation.
• Development of a ‘metabolomics’ approach to build profiles for epoxide and diol products as a tool to assist the investigation of numerous pathophysiological states.

Publications

34. Shearer, G.C., J.W. Newman, B.D. Hammock, G.A. Kaysen. The graded effects of proteinuria on HDL structure in nephrotic rats. J. Am. Soc. Nephrol. Accepted Jan. 2005. [PDF]

33. Newman, J.W., C. Morisseau, B.D. Hammock. 2005. Epoxide hydrolases: Their role and interactions with lipid metabolism. Prog. Lipid Res. 44:1-51. [PDF]

32. Newman J.W., Stok J.E., Vidal J.D., Corbin C.J., Huang Q., Hammock B.D., Conley AJ. 2004. Cytochrome p450-dependent lipid metabolism in preovulatory follicles. Endocrinology 145:5097-105. [PDF]

31. Seubert, J., B. Yang, A. Bradbury, J. Graves, L. Miller, S. Gabel, R. Gooch, J. Foley, J. Newman, L. Mao, H.A. Rockman, B.D. Hammock, E. Murphy, D.C. Zeldin. 2004. Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway. Circ Res 95:506-14. [PDF]

30. Dey A., Williams R.S., Pollock D.M., Stepp D.W., Newman J.W., Hammock B.D., Imig J.D. 2004. Altered kidney CYP2C and cyclooxygenase-2 levels are associated with obesity-related albuminuria. Obes Res 12:1278-89. [PDF]

29. Zhao, X., T. Yammamoto, J.W. Newman, I-H. Kim, T. Watanabe, B.D. Hammock, J. Stewart, J.S. Pollock, D.M. Pollock, J.D. Imig. 2004. Soluble epoxide hydrolase inhibition protects the kidney from hypertension-induced damage. J. Am. Soc. Nephrol. 15:1244-1253. [PDF]

28. DuTeaux, S.B., J.W. Newman, C. Morisseau, E.A. Fairbairn, K. Jelks, B.D. Hammock, M.G. Miller. 2004. Epoxide hydrolases in the rat epididymis: Possible roles in xenobiotic metabolism and endogenous fatty acid metabolism. Toxicol. Sci. 78: 187-195. [PDF]

27. Sather, P.J., J.W. Newman, M.G. Ikonomou. 2003. Congener based Aroclor quantification and speciation techniques – A comparison of the strengths, weaknesses, and proper use of two alternative approaches. Environ. Toxicol. Chem. 37: 5678-5686. [PDF]

26. Viswanathan, S., B.D. Hammock, J.W. Newman, P. Meerarani, M. Toborek, B.Hennig. 2003. Involvement of CYP 2C9 in mediating the proinflammatory effects of linoleic acid in vascular endothelial cells. J. Am. Coll. Nutr. 22(6):502-510. [PDF]

25. Koivunen, M.E., C. Morisseau, J.W. Newman, W.R. Horwath, B.D. Hammock. 2003. Purification and characterization of a methylene urea-hydrolyzing enzyme from Rhizobium radiobacter (Agrobacterium tumefaciens). Soil Biol. Biochem. 35: 1433-1442. [PDF]

24. Watanabe, T., C. Morisseau, J.W. Newman, B.D. Hammock. 2003. In vitro metabolism of the mammalian soluble epoxide hydrolase inhibitor 1-cyclohexyl-3-dodecyl-urea. Drug Metab. Dispos. 31(7): 846-853. [PDF]

23. Newman, J.W., C. Morisseau, T.R. Harris, B.D. Hammock. 2003. The soluble epoxide hydrolase encoded by EPXH2 is a bifunctional enzyme with novel lipid phosphate phosphatase activity. Proc Nat Acad Sci USA. 100(4):1558-63. [PDF]

22. Newman, J.W., T. Watanabe, B.D. Hammock. 2002. The simultaneous quantification of cytochrome P450 dependent linoleate and arachidonate metabolites in urine by high-performance liquid chromatography - tandem mass spectroscopy. J Lipid Res. 43:1563-1578. [PDF]

21. Wheelock, C.E., T.A. Baumgartner, J.W. Newman, M.F. Wolfe, R.S. Tjeerdema. 2002. Effect of nutritional state on Hsp60 levels in the rotifer Brachionus plicatilis following toxicant exposure. Aquatic Toxicol. 61(1-2):89-93. [PDF]

20. Morisseau, C., M.H. Goodrow, J.W. Newman, C.E. Wheelock, D.L. Dowdy, B.D. Hammock. 2002. Structural refinement of urea based soluble epoxide hydrolase inhibitors. Biochem. Pharmacol. 63(9):1599-1608. [PDF]

19. Watkins, S.M., B.D. Hammock, J.W. Newman, J.B. German. 2001. Individual metabolism should guide agriculture toward foods for improved health and nutrition. Am J Clin Nutr. 74(3):283-6. [PDF]

18. Newman, J.W., and B.D. Hammock. 2001 Optimized thiol derivatizing reagent for the mass spectral analysis of disubstituted epoxy fatty acids. J Chromatogr A. 925(1-2):223-40. [PDF]

17. Hunt, J.W., B.S. Anderson, B.M. Phillips, J. Newman, R.S. Tjeerdema, R. Fairey, H.M. Puckett, M. Stephenson, R.W. Smith, and C.J. Wilson. 2001. Evaluation and use of sediment toxicity reference sites for statistical comparisons in regional assessments. Environ. Toxicol. Chem. 20(6):1266-1275. [PDF]

16. Morisseau, C., J.W. Newman, D.L. Dowdy, M.H. Goodrow and B.D. Hammock. 2001. Inhibition of microsomal epoxide hydrolases by ureas, amides and amines. Chem. Res. Toxicol. 14:409-415. [PDF]

15. Slim, R., B.D. Hammock, M. Toborek, L.W. Robertson, J.W. Newman, C.H.P. Morisseau, B.A. Watkins,V. Saraswathi, and B. Hennig. 2001. The role of methyl-linoleic acid epoxide and diol metabolites in the amplified toxicity of linoleic acid and polychlorinated biphenyls to vascular endothelial cells. Toxicol. Appl. Pharmacol. 171(3):184-193. [PDF]

14. Anderson, B.S., J.W. Hunt, B.M. Phillips, R. Fairey, H.M. Puckett, M. Stephenson, K. Taberski, J. Newman, R.S. Tjeerdema. 2001. Influence of sample manipulation on contaminant flux and toxicity at the sediment-water interface. Mar. Environ. Res. April, 2001. 51(3):191-211. [PDF]

13. Newman, J.W., D.L. Denton, C. Morisseau, C.S. Koger, C.E. Wheelock, D.E. Hinton and B.D. Hammock. 2001. Evaluation of fish models of soluble epoxide hydrolase inhibition. Environ Health Persp. 109(1):61-66. [PDF]

12. Yu, Z., F. Xu, L.M. Huse, C. Morisseau, A.J. Draper, J.W. Newman, C. Parker, L. Graham, M.M. Engler, B.D. Hammock, D.C. Zeldin and D.L. Kroetz. 2000. Soluble epoxide hydrolase regulates hydrolysis of vasoactive epoxyeicosatrienoic acids. Circ. Res. 87(11):992-998. [PDF]

11. Morisseau, C., J.K. Beetham, F. Pinot, S. Debernard, J.W. Newman and B.D. Hammock. 2000. Cress and potato soluble epoxide hydrolases: Purification, biochemical characterization, and comparison to mammalian enzymes. Arch. Biochem. Biophys. 378(2):321-332. [PDF]

10. Greene, J.F., J.W. Newman, K.C. Williamson, and B.D. Hammock. 2000. Toxicity of epoxy fatty acids and related compounds to cells expressing human soluble epoxide hydrolase. Chem. Res. Toxicol. 13(4):217-226. [PDF]

9. Greene, J.F., K.C. Williamson, J.W. Newman, C. Morisseau, and B.D. Hammock. 2000. Metabolism of monoepoxides of methyl linoleate: bioactivation and detoxification. Arch. Biochem. Biophys. 376(2):420-432. [PDF]

8. Newman, J.W., J.S. Becker, G. Blondina, and R.S. Tjeerdema. 1999. Quantitation of Aroclors using congener specific results. Environ. Toxicol. Chem. 17(11):2159-2167. [PDF]

7. Morisseau, C., G. Du, J.W. Newman, and B. D. Hammock. 1998. Mechanism of mammalian soluble epoxide hydrolase inhibition by chalcone oxide derivatives. Arch. Biochem. Biophys. 356(2):214-228. [PDF]

6. Fairey R., C. Roberts, M. Jacobi, S. Lamerdin , R. Clark , J. Downing, E. Long, J. Hunt, B. Anderson, J. Newman, R. Tjeerdema, M. Stephenson, and C. Wilson. 1998. Assessment of sediment toxicity and chemical concentrations in the San Diego Bay Region, California, USA. Environ. Toxicol. Chem. 17(8):1570-1581. [PDF]

5. Anderson, B.S., J.W. Hunt, B.M. Phillips, S. Tudor, R. Fairey, J. Newman, H.M. Puckett, M. Stephenson, E.R. Long, R.S. Tjeerdema. 1998. Comparison of marine sediment toxicity test protocols for the amphipod Rhepoxynius abronius and the polychaete worm Nereis (Neanthes) arenaceodentata. Environ. Toxicol. Chem. 17(5):859-866. [PDF]

4. Fairey R., K. Taberski , S. Lamerdin , E. Johnson , R.P. Clark , J.W. Downing, J. Newman and M. Petreas. 1997. Organochlorines and other environmental contaminants in muscle tissue of sportfish collected from San Francisco Bay. Mar. Pollut. Bull. 34: 1058-1071. [PDF]

3. Beckmen, K.B., L.J. Lowenstine, J. Newman, J. Hill, K. Hanni, and J. Gerber. 1997. Clinical and pathological characterization of northern elephant seal skin disease. J. Wildlife Dis. 33(3):438-449. [PDF]

2. Hunter, C. L., M.D. Stephenson, R.S. Tjeerdema, D.G. Crosby, G.S. Ichikawa, J.D. Goetzl, K.M. Paulson, D.B. Crane, M. Martin, and J.W. Newman. 1995. Contaminants in oysters in Kaneohe Bay, Hawaii. Mar. Pollut. Bull. 30: 646-654. [PDF]

1.Newman, J.W., J. Vedder, W.M. Jarman, and R.R. Chang. 1994. A method for the determination of environmental contaminants in living marine mammals using microscale samples of blubber and blood. Chemosphere 28: 1795-1805. [PDF]